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中国癌症防治杂志

中国癌症防治杂志 ›› 2012, Vol. 4 ›› Issue (4): 315-318.doi: 10.3969/j.issn.1674-5671.2012.04.04

• 基础研究 • 上一篇    下一篇

乳腺癌术后放疗患者T淋巴细胞亚群与ER、PR、Her-2表达的相关性研究

  

  1. 甘肃省肿瘤医院放疗科
  • 出版日期:2012-12-25 发布日期:2013-01-11
  • 通讯作者: 高力英 E-mail:lygaolanzhou@yahoo.com.cn
  • 基金资助:

     甘肃省技术研究与开发专项经费资助项目（0912TCYA）

Correlation between T cell subsets and expression of ER，PR，and Her-2 in breast cancer patients after postoperative radiotherapy

  • Online:2012-12-25 Published:2013-01-11

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被引次数

12

摘要： 目的 研究T淋巴细胞亚群在乳腺癌术后患者放疗前、后的变化与ER、PR、Her-2表达之间的相关性，为临床判断预后以及乳腺癌术后治疗提供一定的参考依据。方法 采用流式细胞仪BD FACS Calibur检测46例乳腺癌术后患者静脉血中淋巴细胞亚群，包括T细胞、Th细胞、Ts细胞、NK细胞以及CD4+/CD8+比值，并用四色荧光进行分析。采用免疫组化法检测乳腺癌组织ER、PR、Her-2的表达，了解T淋巴细胞亚群在乳腺癌术后患者放疗前、后的变化与ER、PR、Her-2表达之间的相关性。结果 ER受体的阳性表达与Th细胞密切相关（P=0.000），其在Th细胞于放疗前、后无明显变化组的阳性表达率显著高于在放疗后Th细胞较放疗前降低且低于正常值组的表达率；PR受体的阳性表达与Th细胞密切相关（P=0.014），其在Th细胞于放疗前、后无明显变化组的阳性表达率显著高于在放疗后Th细胞较放疗前降低且低于正常值组的表达率。ER、PR受体和Her-2的表达与放疗前、后的总T细胞、Ts细胞、CD4+/CD8+比值和NK细胞等的变化均无关（P＞0.05）。结论 ER受体或PR受体阴性表达的患者，术后放疗可引起免疫功能较放疗前降低，预后可能较差 。

关键词: 乳腺肿瘤, 放疗, T淋巴细胞亚群, ER, PR, Her-2

Abstract:  Objective To explore the correlation between T cell subsets and expression of ER，PR，and Her-2 in breast cancer patients before and after postoperative radiotherapy， in order to develop markers to guide clinical prognosis and postoperative treatment of breast cancer. Methods Several T cell subsets（T，Th，Ts，NK）and the CD4+/CD8+ ratio in peripheral blood lymphocytes from 46 breast cancer patients were analyzed by standard four color flow cytometry on a BD FACS Calibur. Immunohistochemistry was used to assess expression levels of ER，PR，and Her-2 in breast cancer tissue. The correlation between T cell subsets and expression of ER， PR，and Her-2 in breast cancer patients was analyzed before and after postoperative radiotherapy. Results Expression of the ER receptor and PR receptor， which strongly correlated with Th cells（P=0.000 and 0.014， respectively），was significantly higher in the Th subsets that changed significantly as a result of radiotherapy than in the Th subsets that did not change significantly.No correlation was observed between T cell subsets and expression of ER，PR，or Her-2 before and after postoperative radiotherapy（P>0.05）. Conclusions Patients who are ER- or PR negative may show decreased immune function and poor prognosis after treatment with postoperative radiotherapy.

Key words:  Breast neoplasms, Radiation treatment, T cell subsets, ER, PR, Her-2